Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Longitudinal remodeling of gastric microbiota following Helicobacter pylori eradication reveals an eradication-associated microbial signature in gastric cancer

doi: 10.3389/fcimb.2026.1848437

Figure Lengend Snippet: Predicted functional pathways based on KEGG annotations associated with H. pylori infection and eradication status. (A) Relative representation of predicted pathways between H. pylori –negative and H. pylori –positive samples **, P < 0.01; ***, P < 0.001 for two-group comparison. (B) Relative changes in predicted functional pathways between baseline and follow-up samples across eradicated, recurrent GC after eradication, and non-eradicated groups. Functional profiles were predicted from 16S rRNA gene sequencing data using KEGG-based pathway inference. *, P < 0.05; ***, P < 0.001 for comparison between baseline and follow-up in each group. KEGG, Kyoto Encyclopedia of Genes and Genomes.

Article Snippet: The raw 16S rRNA gene sequencing data generated in this study have been deposited in the National Center for Biotechnology Information Sequence Read Archive under the BioProject accession number PRJNA1447187 and are publicly available at http://www.ncbi.nlm.nih.gov/bioproject/1447187 .

Techniques: Functional Assay, Infection, Comparison, Sequencing

Journal: bioRxiv

Article Title: Taxonomic Composition and Predicted Functional Potential of a Commercial Microbiome-Based Fertilizer Additive and Agricultural Soils in Eastern Paraguay

doi: 10.64898/2026.06.03.729874

Figure Lengend Snippet: Heatmap showing the relative abundance of identified PGPR-associated metabolic pathways across five samples (Root_13, Root_14, Humus_4 (surface), Humus_5 (middle depth), and Humus_6 (deep), inferred from 16S rRNA gene sequencing data using PICRUSt2. Pathways are hierarchically clustered (dendrograms) based on similarity in predicted functional profiles. The color gradient represents normalized relative abundance values, ranging from −1 (blue, lower abundance) to 1.5 (red, higher abundance), with intermediate levels shown in white/yellow.

Article Snippet: 16S rRNA gene sequencing was performed by Azenta Life Sciences (South Plainfield, NJ, USA) using their 16S-EZ service.

Techniques: Sequencing, Functional Assay

Journal: Frontiers in Microbiology

Article Title: Development of a mixed microbial culture for robust high-throughput ex-situ thermophilic biomethanation

doi: 10.3389/fmicb.2026.1840981

Figure Lengend Snippet: qPCR results displaying gene copy numbers per mL for bacteria (a) , Methanobacteria (b) , Methanosarcina (c) , and Methanosaeta (d) in untreated (blue columns) and autoclaved (orange columns) anaerobic digestate. The standard error of the mean was generally within 5% of the mean across all measurements ( n = 3). (e) 16S rRNA analysis illustrating the relative abundance of microbial phyla in untreated anaerobic digestate. (f) 16S rRNA analysis showing the relative abundance of microbial phyla in autoclaved anaerobic digestate.

Article Snippet: High-throughput sequencing was performed on the HiSeq Illumina platform with bacterial (515F/806R) and archaeal (Arch519F/Arch915R) 16S rRNA gene primers (Novogene, UK).

Techniques: Bacteria

Journal: Frontiers in Microbiology

Article Title: Development of a mixed microbial culture for robust high-throughput ex-situ thermophilic biomethanation

doi: 10.3389/fmicb.2026.1840981

Figure Lengend Snippet: 16S rRNA analysis showing the relative abundance of microbial phyla in samples collected on days indicated on the primary horizontal axis.

Article Snippet: High-throughput sequencing was performed on the HiSeq Illumina platform with bacterial (515F/806R) and archaeal (Arch519F/Arch915R) 16S rRNA gene primers (Novogene, UK).

Techniques: